effects of pistacia atlantica subsp. kurdica on growth and aflatoxin production by aspergillus parasiticus

background aflatoxins are highly toxic secondary metabolites mainly produced by aspergillus parasiticus. this species can contaminate a wide range of agricultural commodities, including cereals, peanuts, and crops in the field. in recent years, research on medicinal herbs, such as pistacia atlantica subsp. kurdica, have led to reduced microbial growth, and these herbs also have a particular effect on the production of aflatoxins as carcinogenic compounds. conclusions pistacia atlantica subsp. kurdica has anti-toxic properties in addition to an inhibitory effect on a. parasiticus growth, and is able to decrease aflatoxin production effectively in a dose-dependent manner. therefore, this herbal extract maybe considered a potential anti-mycotoxin agent in medicine or industrial agriculture. results the results showed that p. atlantica subsp. kurdica can inhibit a. parasiticus growth at a concentration of 125 mg/ml. hplc results revealed a significant decrease in aflatoxin production with 125 mg/ml of p. atlantica subsp. kurdica, and afl-b1 production was entirely inhibited. based on quantitative real-time pcr results, the rate of aflr gene expression was significantly decreased after treatment with p. atlantica subsp. kurdica. objectives in this study, we to examine p. atlantica subsp. kurdica as a natural compound used to inhibit the growth of a. parasiticus and to act as an anti-mycotoxin. materials and methods in vitro antifungal susceptibility testing of p. atlantica subsp. kurdica for a. parasiticus was performed according to clsi document m38-a2. the rate of aflatoxin production was determined using the hplc technique after exposure to different concentrations (62.5 - 125 mg/ml) of the gum. the changes in expression levels of the aflr gene were analyzed with a quantitative real-time pcr assay.